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. 2011 Jul 14;6(7):e21011. doi: 10.1371/journal.pone.0021011

Figure 3. ILT2-mediated signaling by B2M-HLA-G5 and B2M-HLA-G1s-Fc proteins.

Figure 3

NFAT-GFP reporter cells expressing the ILT2-PILRβ chimera were stimulated for 16 h with 1.5 µg/ml of the indicated HLA-G recombinant proteins. Non-treated reporter cells were used as negative control, and tetrameric complexes of HLA-G1 (HLA-G1t, [31]) were used as positive control. GFP expression on reporter cells was analyzed by flow cytometry. Numbers indicate the percentage of GFP-positive cells. Data shown are from one of four independent experiments.