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. 2011 Jul 14;7(7):e1002123. doi: 10.1371/journal.ppat.1002123

Figure 2. SIM deletion mutants of PML are defective in recruitment to sites associated with HSV-1 genomes.

Figure 2

A. Maps of PML isoforms I and IV and derivatives lacking exon 7a. B. Western blot of the EYFP-linked proteins in control and PML depleted cells using an anti-EGFP antibody. The major unmodified bands of the EYFP-PML proteins are indicated by asterisks. C. Localization of EYFP-PML proteins (green) and endogenous Sp100 (red) in uninfected control and PML depleted cells. D. Typical assays of recruitment of EYFP-proteins (green) to sites associated with HSV-1 genomes (ICP4; red) in ICP0 null mutant (ΔICP0) infected control and PML depleted cells. Scale bars indicate 5 µm.