Figure 6. Recombination of hopM1 in group I strains leads to functional divergence.

(A) A genomic region including hopM1 was aligned for all group I P. syringae strains with draft genome sequences and pairwise nucleotide diversity values (π) were calculated in 25 bp sliding windows. (B) Phylogenies were constructed using Bayesian methods for both avrE1 and hopM1 for all publicly available alleles. Posterior probabilities are shown if support for nodes is <0.99. Color-coding of strain names represents phylogenetic group designation as described in Figure 1. (C) schM/hopM1 from Pmp is unable to complement the virulence defect of Pto DC3000 ΔCEL in dip assays on Arabidopsis. Bars indicate mean growth at zero and four days after inoculation. Error bars are 2× standard error. Different letters indicate statistically significant differences (ANOVA, Tukey's HSD).