Skip to main content
. 2011 Jul 14;6(7):e21968. doi: 10.1371/journal.pone.0021968

Figure 6. CD103 expression is not required for cytokine production by gut infiltrating CD8 T cells.

Figure 6

Equal numbers of CD8 T cells from BALB/c-WT (WT, Thy1.1+Thy1.2-) and BALB/c-CD103 KO donors (KO, Thy1.1+Thy1.2+) were adoptively transferred into a group (n = 3) of lethally irradiated A/J mice together with WT BMC plus SaI/N tumor cells. Lymphocytes were isolated from the host intestinal epithelium at day 7 post-transplant and subjected to intracellular staining for INFγ, TNFα and IL-2. A. Representative histograms of cytokine staining by gated CD8+ lymphocytes of either WT or KO origin (thin line) vs. isotype control staining (heavy line). B. Mean production (±SEM) of the indicated cytokines by gated WT (n = 3, solid bars) or KO CD8 T cells (n = 3, open bars). Data represent two independent experiments with similar results.