Table 1. Primers and strains used in the study.
Primers | Sequences (5′→3′) |
FP1s | TTTTAGCCTGCCCATCCC |
FP1as | CCCAGCACCTCCAGAATCA |
FP2s | GGGACAGAGGGTATTGTTTTG |
FP2as | ATCCTGGCCGAGATGCTT |
PMKO1 | ATGGCAGCCGTTGTAGCTCT |
PMKO2 | TTAAGCAGTGGAGTGGAAGCT |
CFg1 | CCATCGATGGCAGCCGTTGTAGCTCTAC a |
CFg2 | CGCGGATCCTTAAGCAGTGGAGTGGAA b |
CMo1 | CAGATCTTGGCTTTCGTAGGAACCCAATCTTCAATGGCAGCCGTTGTAGCTC |
CMo2 | CACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACAGCAGTGGAGTGGAAGCT |
PKQF | CGCCTCTACTACTTCAACTACTGCC |
PKQR | CCCTCGCCGATAACATCCATAAC |
PsEFQF | TTCGCCGTCCGTGATATGAGACAA |
PsEFQR | ATGCGTATCATGGTGGTGGAGTGA |
The ClaI and BamHI sites introduced in the primer sequences were underlined.