Figure 1. Quenching of tcM5 activity by inhibitors in plasmas from different species.

A. tcM5 (10 µg/mL) incubated (37°C, 1 h) in plasma and activity monitored with uPA enzymatic substrate (S2244). Rat plasma induced little tcM5 inhibition, in contrast to the other plasmas. B. After 1 h of incubation, each plasma sample was examined by zymography. No tcM5 complex with C1I, and only a faint complex with AT formed in rat plasma, in contrast to the other species.