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. 2011 Jul 14;6(7):e22225. doi: 10.1371/journal.pone.0022225

Figure 3. In vivo microdialysis in mouse ISF.

Figure 3

(A) α-Synuclein concentration in ISF of Tg mice is stable 6 hours after probe implantation. Probe was allowed to equilibrate in mouse striatum for 2 hours and three microdialysate samples were collected at 2 hour-intervals at a flow rate of 0.6 µl/min. α-Synuclein concentration in the microdialysate samples was determined by ELISA. Stable levels of α-synuclein were recovered only 6 hours following probe insertion (n = 3, mean ± SD, one way ANOVA test followed by Tukey's test, ** p<0.01). (B) The levels of α-synuclein in mouse ISF remain constant over a period of 3 days. Probe was allowed to equilibrate for 4 hours before fraction collection. α-Synuclein concentration in the microdialysates was measured by ELISA. (C) In vivo concentration of α-synuclein in the ISF of knock-out (KO), wild type (WT) and transgenic (Tg) mice. Microdialysis was performed as described in the Methods section. α-Synuclein concentration was determined by ELISA 6 hours following probe insertion. α-Synuclein levels were significantly increased in Tg mice (0.49±0.27 ng/ml, n = 14) compared to the WT mice (0.15±0.12 ng/ml, n = 9). Data are presented as mean ± SD and statistics were performed by one way ANOVA test followed by Tukey's test (** p<0.01). As expected, α-synuclein was not detected in the microdialysates of KO mice (n = 5).