Table 1. Total number of similar amino acid sites between the different groups of sarcopterygians which have been affected by positive-destabilizing during cladogenesis.

	A-L	A-C	L-C	A-L-C
pK'	117	26	6	0
Ra	157	19	7	0
an	2	17	0	17
Hp	14	9	0	0
H	16	0	1	3
Ko	62	2	3	2
F	64	13	0	5
Ht	27	2	0	0
P	15	16	0	3

Abbreviations: A- Amphibians, L- Lungfishes, C- Coelacanths; (pK') - Equilibrium constant (ionization of COOH), (R_a) - Solvent accessible reduction ratio, (a_n) - Power to be at the N-terminal, (H_p) - Surrounding hydrophobicity, (H) - Hydropathy, (K^o) - Compressibility, (F) - Mean r.m.s. fluctuation displacement, (H_t) - Thermodynamic transfer hydrophobicity, (P) – Polarity. The table shows clearly that the total number of amino acid sites commonly affected by positive selection is much higher between amphibians and lungfishes (A-L) when compared to the other groupings. Hence we come to a conclusion that amphibians and lungfishes are very closely related. Also pK' and R_a have affected the genes to a maximum extent unlike other properties. Please note that only the major amino acid properties are listed in the table (Refer to Table S2 for all the details).