Figure 4. EMCV infection of Hela cells depleted or not of eIF2α.
A) Hela cells transfected with a mixture of siRNAs targeting eIF2α mRNA or mock Hela cells were infected with EMCV (10 pfu/cell) at 36 h post-transfection. Next, protein synthesis was determined by [35S]Met-Cys labelling at the hpi indicated. Samples were analyzed by SDS-PAGE, fluorography and autoradiography. B) Western blot analysis of samples from panel A using anti-eIF2α or anti-phospho-eIF2α antibodies. As a control, the amount of eIF4GI in each sample was determined using specific antibodies against this factor. The arrows indicate viral proteins.