Figure 8. Translation of Mengovirus and PV replicons. Effect of Ars on early and late viral protein synthesis.

A) BHK cells were electroporated with Mengo-luc, Polio-luc replicons, Cap-luc or CrPV IGR-luc mRNAs; all these RNAs were synthesized by in vitro transcription. Electroporated cells were seeded in DMEM (10% FCS) in presence or absence of 200 µM Ars. 75 min later cells were collected and lysed to measure luc activity. The values shown are percentages of the value of their respective Ars untreated samples and are means ± SD of three independent experiments. B) The phosphorylated form of eIF2α and total eIF2α were determined in parallel by Western blot with specific antibodies. C and D) BHK cells were electroporated with Mengo-luc replicon (C) or polio-luc replicon (D) and at 5 h post-electroporation protein synthesis was determined by [³⁵S]Met-Cys labelling in presence of different concentrations of Ars (0, 50, 100 and 200 µM). The arrows indicate viral proteins.