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. 2011 Jul 14;6(7):e22217. doi: 10.1371/journal.pone.0022217

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Bolte et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Whole-heart RNA was isolated from embryonic Nkx2.5-Cre/Foxm1^fl/fl and control Foxm1^fl/fl hearts and used for qRT-PCR analysis. Taqman primers specific for CaMKIIδ, β-catenin, NFATc3, GATA4, GATA 6, Hey2, myocardin, Twist1, Twist2, Sox4, Notch1, Notch4, FGF10, IL-1β, TGF-β₁, BMP2, BMP4, Wnt5a and Wnt7b (Table 1) were used to evaluate cardiac mRNA expression. Gene expression was normalized to β-actin mRNA. Significantly diminished mRNA levels of the intracellular signaling molecule CaMKIIδ and transcription factors NFATc3, Hey2 and myocardin were detected in Nkx2.5-Cre/Foxm1^fl/fl hearts. Increased mRNA expression of IL-1β was also detected. Significant differences (p<0.05) were indicated by asterisk. “N” values were represented by boxes inside bars.