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. 2011 Jul 14;6(7):e22253. doi: 10.1371/journal.pone.0022253

Figure 4. Time trajectories of the fluorescence intensity and calculated fluorophore angles.

Figure 4

(A, C) Time trajectory of BSR intensity in the absence (A) and presence of caged ATP (C). The UV flash occurred at 30 s (arrow). The trajectories of BSR intensities after subtracting the background signals were averaged over every five frames, and fitted to a quadratic cosine function (solid lines). Black, fluorescence intensity before UV flash; blue, fluorescence intensity after UV flash. (B, D) Distributions of Δθ in the absence (B) and presence of caged ATP (D). Positive angle, angular displacement measured counterclockwise; negative angle, angular displacement measured clockwise. In the absence of caged ATP (B), the distribution corresponded to a Gaussian distribution with a peak of −0.42°. In the presence of caged ATP (D), the distribution can be fitted by a sum of three Gaussian functions, with peaks of 1.8°, 34°, and −35°.