Skip to main content
. 2011 Jul 14;6(7):e22246. doi: 10.1371/journal.pone.0022246

Figure 6. Osmotic response of TRPV1 is potentiated under acidic conditions.

Figure 6

(A) Representative single cell traces of the fluorescence ratio during the perfusion of solutions with the respective osmolality at 36°C. The top line indicates the timing of the change from the control solution (pH 7.4, 300 mOsm; gray) to the acidic solution (pH 6.6) with the respective osmolality (black). (B) Summary of the change in the fluorescence ratio. Data are differences between fluorescence ratios 2 min before and 4 min after the change of the solution. The osmotic response of the [Ca2+]i in HEK293-TRPV1 cells (filled circles) was enhanced by acidification over the entire osmolality range (compare with filled triangles). This was evident especially in the hypertonic range. Values are the mean ± SEM; when the SEM value was less than 0.07, the error bar is hidden behind the symbol. HEK293-TRPV1, pH 7.4: n = 138 (250 mOsm), n = 151 (300 mOsm), n = 214 (350 mOsm). HEK293-TRPV1, pH 6.6: n = 151 (250 mOsm), n = 144 (300 mOsm), n = 251 (350 mOsm). HEK293, pH 6.6: n = 129 (250 mOsm), n = 131 (300 mOsm), n = 156 (350 mOsm).