Figure 2. The class I PI3K regulatory subunits p85α and p85ß are not required for SopB-mediated Akt phosphorylation.

HeLa cells were transfected with siRNAs, specific for p85α and p85ß, for 72 hr then either treated with EGF or infected with Salmonella WT. For siRNA control siRNA specific for AKT3 was used (cont.). Monolayers were then solubilized in sample buffer and processed for immunoblotting using antibodies to detect phospho Akt (Ser473), total Akt or p85α. (A) Representative immunoblot showing p85α knockdown efficiacy and effect on Akt phosphorylation in infected or EGF treated cells. (B) Quantification of Akt phosphorylation estimated by densitometry. Shown are the means ± SD from three independent experiments.