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. 2011 Jul 14;6(7):e22260. doi: 10.1371/journal.pone.0022260

Figure 4. Both PDK1 and rictor are required for Salmonella-induced Akt phosphorylation.

Figure 4

A and B. HeLa cells were transfected with the indicated SMART pool siRNA for 48 hr then infected with Salmonella WT or ΔsopB. For siRNA control SMART pool siRNA specific for AKT3 was used (cont.). After 30 min, monolayers were solubilized and processed for immunoblotting. Antibodies were used to detect phospho-Akt (Ser473 or Thr308), total Akt, PDK1, raptor, rictor or actin. Gray bars underneath the individual panels highlight the efficiency of each siRNA knockdown.