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. 2011 Jul 14;6(7):e22260. doi: 10.1371/journal.pone.0022260

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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HeLa cells were transfected with plasmids encoding epitope-tagged proteins as indicated. After 20 hr they were infected with Salmonella for 30 min, then fixed and processed for immunofluorescence microscopy. GFP-HA-Akt transfected cells were stained for Salmonella LPS (Cy5) and phospho Akt Ser473 (AF568: A–C), Myc (AF568: D–F) or 3×FLAG (AF568: J–L). Cells expressing PDK1-Myc (G–I) were stained for LPS (Cy5), Myc (AF488) and actin filaments (phalloidin-AF568) to reveal the ruffles.