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Primers sequences and annealing conditions for the Fas signaling molecules
| Gene target |
Annealing T (°C) |
Forward primer | Reverse primer |
|---|---|---|---|
| Fas | 59 | GGCTATAGATCACCTTCATGTA | GCAGTTAACTCAGGGACCAAG |
| FADD | 50 | AGGCATCTACACAGCCTGGACTTT | CCGCTGCCTTGGCAATTCTGTTAT |
| beta-actin | 49-60 | ATCATGTTTGAGACCTTCAACA3 | CATCTCTTGCTCGAAGTCCA |
