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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Cancer. 2011 Mar 1;117(17):4092–4099. doi: 10.1002/cncr.26021

Figure 4. MNCs are more resistant to adriamycin than are mononuclear cells.

Figure 4

A–D, TUNEL assay of UV-2237 cells treated with adriamycin (10 µM for 24 h). MNCs are indicated by arrows, mononuclear cells are indicated by arrowheads. TUNEL positive signals are in green and propidium iodide (PI) counterstained nuclei are in red. The percentage of positive TUNEL cells within mononuclear cells were 74.4+/-8.1%, and none TUNEL positive cells were fund in the MNCs. Bar = 50µm. E and F, At the 72 hr after adriamycin treatment, when the dead cells were washed off from the culture dish, all the remaining live cells were found to be MNCs (nucleus indicated by arrows).Bar=50µm.