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Native ESI and ECD/CID top-down spectra of the FMO antenna protein complex. (a–c) native ESI spectra of FMO with 0, 10, 20 V ISCID. No fragment ions were observed. (d) ECD spectrum of FMO protein with 15 V ISCID. (e) CID (10 V) spectrum of FMO; no fragment ions were observed. (f) CID (20 V) spectrum of FMO. Multiply charged fragment ions were observed at low m/z.
