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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Oncogene. 2011 Mar 7;30(28):3163–3173. doi: 10.1038/onc.2011.39

Figure 4.

Figure 4

KiSS1 is lost in metastatic HNSCC tumors. (a) Real-time (RT)–PCR analysis of KiSS1 and GAPDH in metastatic and nonmetastatic human HNSCC tumors, showing decreased expression or loss of KiSS1 in the metastatic tumors. Specimens run on different gels are separated by a black vertical line. (b) KiSS1 densitometric expression in human tumors from (a) were normalized to GAPDH levels, and data were averaged and analyzed with Student's t-test. (c) CDDP-resistant cell lines were transfected with either an empty vector (pcDNA3-EV) or a vector containing the full-length KiSS1 coding sequence. The expression of KiSS1 was analyzed by SDS–PAGE (western blot, top panel) and real-time RT–PCR (PCR, bottom panel). (d) Mock- or KiSS1-tranfected cells were seeded onto six-well plates, grown to confluency and assessed for haptotaxis after a scratch wound was made. Images were taken at 0, 12 and 24 h, and the degree of wound closure was determined with ImagePro. Experiments were performed in triplicate and repeated three times. Columns represent percentage of wound closure from 0 h; bars represent s.e.m.; magnification × 100. A full colour version of this figure is available at the Oncogene journal online.