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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Hepatol. 2010 Dec 13;55(2):399–406. doi: 10.1016/j.jhep.2010.11.025

Fig.3. Transcriptional response elements in the CTGF promoter that drive ethanol-stimulated reporter activity in LX-2 cells.

Fig.3

(A) Organization of CTGF promoter (−805 to +17), fused to a SEAP reporter gene showing the location of key transcriptional elements that were altered by point mutation or deletion mutation. (B) Cultured LX-2 cells were transfected with plasmids containing the CTGF promoter-reporter, serum-starved for 18h, and then treated with (i) 0–20 ng/ml TGF-β1 for 6h (left) or 10 ng/ml TGF-β1 for 0–240 mins (right) or (ii) 0–50 mM ethanol for 2h (left) or 25 mM ethanol for 0–240 mins (right). SEAP reporter activity was calculated after adjustment for differences among samples in transfection efficiency as determined by co-transfection with a β-gal reporter. (**p<0.001, +p<0.05 v no treatment). (C) LX-2 cells were transfected with wild type or mutant CTGF promoter reporters after which cells were treated with 0 or 25mM ethanol for 48h. SEAP reporter activity was calculated after adjustment for differences among samples in transfection efficiency as determined by co-transfection with a β-gal reporter. (**p<0.001 v FL alone; # # p<0.001 v FL + ethanol).