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. 2008 Dec 29;18(8):1155–1166. doi: 10.1089/scd.2008.0391

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 2. — The side population (SP) displays characteristic markers of undifferentiated embryonic stem cells (ESCs) and distinct expression pattern of early development genes. (A–H) Phenotypic characterization of the SP fraction by FACS. (A–D) ESC antigenic markers. (E–F) Adult stem cell antigenic markers. Emission peaks for the indicated antigen and Isotype controls are shown. Y-axis: normalized frequency of events; X-axis: fluorescence intensity for the indicated antigen. (I) Comparative QRT-PCR analysis of early embryo development markers. Y-axis: gene expression level in the SP fraction relative to the non-SP fraction (fold change). Values plotted as the average ± standard error. Results from at least three independent experiments. Statistically significant differences (P < 0.05) are denoted as “*”. (J) Comparative analysis of alkaline phosphatase activity for the SP and non-SP fractions. Y-axis: phosphatase activity as absorbance of enzymatic product in 20,000 FAC sorted cells. Activity was measured on a spectrophotometer by reading amount of product produced after catalytic colorimetric reaction. Values plotted as the average ± standard error. Results from three independent experiments. *P < 0.05.