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. 2008 Dec 29;18(8):1155–1166. doi: 10.1089/scd.2008.0391

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 4. — Reversibility between side population (SP) and non-SP embryonic stem cell (ESC) populations. (A–E) Indirect immunofluorescence microscopy analysis of SSEA1 expression in cultures derived from non-SP cells. Scale bar, 100 μm. (E–F) Representative Hoechst profile of non-SP derived culture at passages 2 and 4. (G–H) Hoechst analysis of single-cell-derived cultures. (G): SP-derived culture. (H): Non-SP-derived culture. Y-axis: Hoechst red emission; X-axis: Hoechst blue emission. SP gate is shown in each panel.