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Immunophenotype of freshly isolated (FI) and passaged (P0–P5) EMSCs. (A) Hematopoietic markers CD4 and CD45. (B) Stromal markers CD90, CD73, and CD44. (C) Stem cell markers CD117 and Sca-1. All values reflect the mean ± standard deviation. Asterisks indicate significant differences between freshly isolated and passaged cells (*P < 0.05; ***P < 0.001). Abbreviation: N/A, non-analyzed.
