Factors influencing EPZ. (A–D) Relationship of EPL to TF length, wall shear rate, TF density, and TM density, respectively. For A–C, TFlength is 2 mm, wall shear rate is 500 s−1, [TF] is 1 fmol/cm2, and [TM] is 1000 fmol/cm2 when fixed. In B, EPH is shown as a percentage of total chamber height. In D, [TF] is 0.8 fmol/cm2, TFlength is 2 mm, and wall shear rate is 500 s−1.