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. 2011 Jul 11;6(7):e21301. doi: 10.1371/journal.pone.0021301

Figure 2. GhRDL1 expression patterns in cotton and GFP:GhRDL1 and GhMYB2:YFP transgene expression in A. thaliana Col and try mutant transgenic plants.

Figure 2

(A) Quantitative RT PCR (qRT-PCR) analysis of GhRDL1 expression in ovules, fibers, and non-fiber tissues of TM-1 and ovules (0 DPA) of N1N1. (B–C) RNA in situ hybridization in cotton ovules (0 DPA) using sense (B) and anti-sense (C) probes showing accumulation of GhRDL1 transcripts in fiber cell initials (C). (D) RT-PCR analysis of transgene expression in 35S:GFP:GhRDL1 transgenics, 35S:GhMYB2:YFP transgenics, and 35S:GFP:GhRDL1 and 35S:GhMYB2:YFP double transgenics. Amplification of Tublin (beta 6) gene (TUB) was used as an RNA loading control. (E) RT-PCR analysis of the transgene expression as in (D), except that the transgenic plants were in the try mutant background.