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. 2011 Jul 11;6(7):e21301. doi: 10.1371/journal.pone.0021301

Figure 4. Seed hair production in the transgenic plants overexpressing GhRDL1 alone or GhRDL1 and GhMYB2 together.

Figure 4

(A–D) Initiation of seed hair in the try transgenic plants (seeds) expressing 35S:GFP:GhRDL1 alone. Scanning electron microscope (SEM) images of developing seeds at 13 days after pollination (DAP) (A), 15 DAP (B), 17 DAP (C), and 21 DAP (D). Hair-like initial was observed as early as 11 DAP. The boxed areas were zoomed in (E–H). Scale bar represents 100 µm. (E–H) Enlarged SEM images of corresponding boxed regions in (A–D). Scale bar represents 10 µm. (I–J) A single seed hair in a mature seed observed using light microscope (I) and SEM (J). (K) Multiple seed hairs (two shown) produced in a seed of the transgenic plants overexpressing both GhRDL1 and GhMYB2. (L–M) Seed hair without staining (L) and PI staining (M) in GhRDL1 transgenic plants. PI stained cell walls of seed coat and seed hair. Scale bars in (I–M) represent 50 µm. Arrows in (E–M) indicate seed hair or fiber.