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. 2011 Jul 11;6(7):e21453. doi: 10.1371/journal.pone.0021453

Figure 2. The effect of ApoE on lipoprotein-induced ABCA1 promoter activity.

Figure 2

Pane A: Two constructs containing wild-type and Sp1-binding site-mutated (Mut-Sp1) ABCA1 promoter region were subcloned into a luciferase (Luc) reporter plasmid (pGL-2). The mutated sequences in the Mut-Sp1 construct are shown in the schematic diagram. Panel B: Mouse macrophages were transfected with a β-galactosidase expression plasmid and the ABCA1 promoter-reporter constructs or the empty pGL-2 vector. The transfected macrophages were treated with 20 µg/ml of wild-type (E+/B), ApoE-free (EÎ/B) or ApoE3-enriched (E3/B) lipoproteins, or culture medium alone (control) for 4 hrs. Luciferase activity was normalized to the β-galactosidase activity and expressed relative to that of pGL2 basic vector. Values represent the mean ± SEM of five independent experiments. * P<0.05 compared to cells transfected with the same plasmid and without lipoprotein treatment (control), † P<0.05 compared to cells transfected with the same plasmid and treated with E+/B or E3/B lipoproteins, and # P<0.05 compared to cells transfected with wild-type ABCA1 promoter and treated with the same lipoproteins.