Figure 6. Simulation results for a continuous increase in ROS levels over time.

The rate of ROS production was set to be an increasing function of time. One typical simulation showing a cell which underwent apoptosis at time t = 32.3 h. Note that the curves flatten after cell death as all reactions rates are set to zero. A Native protein, total misfolded protein (includes misfolded bound by Hsps), and reactive oxygen species (ROS). ROS are scaled x100 to allow easier visualisation. B Total Hsp90 (free pools plus all complexes), Free Hsp90 (unbound Hsp90) and Hsp90_MisP (Hsp90 bound to misfolded protein). C Total Hsp70 (free pools plus all complexes), Free Hsp70 (unbound Hsp70) and Hsp70_MisP (Hsp70 bound to misfolded protein).