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. 2011 Aug;52(8):1517–1525. doi: 10.1194/jlr.M014654

Fig. 2.

Fig. 2.

miR-296-5p negatively modulates PUMA expression. A: miR-296-5p levels were increased by stable transfection of Huh-7 cells (WT) with miR-296-5p expression vector (miR-296) and expressed in relation to the small housekeeping RNA Z30. B: PUMA mRNA was measured in WT and miR-296 stable cells by real-time PCR and expressed in relation to the housekeeping rRNA 18S. C: Immunoblot analysis was performed for PUMA and Bim protein expression in WT or miR-296 stable cells. β-actin was used as a control for protein loading. D: miR-296-5p function was inhibited by transient transfection for 24 h with a locked nucleic acid oligonucleotide specific to miR-296-5p (LNA). Scramble LNA were used as control. PUMA mRNA levels were increased in LNA-transfected cells. E: LNA-transfected as in panel D also resulted in significantly higher PUMA protein expression. Data represent the mean and standard error of three experiments; *P < 0.05.