FIGURE 5.

Heparan sulfate proteoglycan content of cell lines. CHO cells, MC38 colon cancer cells, LS174T colon cancer cells, AML12-hepatocye-derived cells, and HepG2 hepatoma-derived cells were trypsinized extensively to remove HSPGs from the cell surface. The cells were then washed and counted, and the heparan sulfate concentrations in both the lysed cell pellets and the cell surface/trypsin-supernatants were determined. The table describes, for heparan sulfate liberated from the cell surface, the percentages of N-, 2-O-, and 6-O-sulfation, and the average number of sulfate groups per disaccharide subunit.