TABLE 3.

Affinity constants of the T. tengcongensis preQ₁ riboswitch and its variants for metabolites

	kon × 104	S.E. × 102	S.D. × 104	koff × 10−4	S.E. × 10−6	S.D. × 10−4	KDa	S.D.	t½	Krel (KD(mut)/KD(WT preQ1)
	m−1s−1			s−1			nm		h
Wild type preQ1	7.77	3.55	3.45	1.53	1.00	0.47	2.05	0.29	1.4	1
Wild type preQ0	0.65	0.27	0.13	2.22	1.04	0.14	35.10	6.07	0.9	17
G11dG preQ1	8.47	2.94	3.71	1.42	0.96	0.58	1.69	0.22	1.6	0.8
G11dG preQ0	0.65	0.20	0.10	1.83	1.07	0.27	28.90	7.7	1.1	14
G5DAP/C16U preQ1	—b	—	—	—	—	—	2.01 × 103	3.32 × 102	—	981
G5DAP/C16U preQ0	—	—	—	—	—	—	1.63 × 103	1.17 × 102	—	795
U6C preQ1	—	—	—	—	—	—	>2.74 × 105	3.05 × 104	—	—
U6C preQ0	—	—	—	—	—	—	>1.99 × 105	2.01 × 104	—	—
A29Pur preQ1	—	—	—	—	—	—	1.84 × 104	3.34 × 103	—	8976
A29Pur preQ0	—	—	—	—	—	—	1.80 × 104	8.42 × 102	—	8781

^a Here we use the nomenclature K_D with the clarification that it is an apparent K_D.

^b —, not determined (apparent K_D obtained from equilibrium analysis) or an estimate that precludes K_rel calculation.