FIGURE 7.

Role of carboxyl terminus of c-Cbl in 5-HT_2AR resensitization. 180 min following a synchronized pulse of 10 μm 5-HT, serum-deprived HEK293 cells transiently co-transfected with 5-HT_2AR and c-Cbl-WT (A), c-Cbl-(1–436) (B), or c-Cbl-N (C) for 24 h were loaded with Calcium 3 dye. The responsiveness of 5-HT_2AR, which had trafficked in the absence (veh) or presence (5-HT) of an agonist, was assessed with fluorometric imaging plate reader by analysis of Ca²⁺ mobilization following exposure to either buffer (untreated) or 1 μm 5-HT (post-pulse). Data shown are representative of seven independent experiments. D, the maximum changes in intracellular Ca²⁺ observed in panels A-C were quantified using GraphPad Prism. Results are mean ± S.E. (n = 7). Asterisks indicate a p of <0.01 versus c-Cbl-WT.