FIGURE 1.

Cell death induced by 24S-OHC in SH-SY5Y and cultured cortical neurons. A, SH-SY5Y cells and primary cortical neuronal cells were treated with variable concentrations of 24S-OHC for 24 h, and the viability was measured by MTT assay, as described under “Experimental Procedures.” **, p < 0.01, when compared with vehicle control (without 24S-OHC). B, SH-SY5Y cells were cultured with 50 μm 24S-OHC for the indicated incubation times, and the viability was measured by MTT assay. **, p < 0.01, when compared with time 0. C, SH-SY5Y cells were treated with 24S-OHC for the indicated times and then cultured in fresh medium for 24 h. Cell viability was measured by LDH assay. **, p < 0.01, when compared with treated time 0 h. N.S., not significant difference when compared with treated time 24 h.