FIGURE 2.

Characterization of cell death induced by 24S-OHC in SH-SY5Y cells. A, SH-SY5Y cells treated with 50 μm 24S-OHC or 1 μm STS for 24 or 7 h were stained with Hoechst 33258 and viewed under a fluorescence microscope. B, cells were treated with 24S-OHC or STS for the indicated times, and the cell samples were then treated with annexin V-FITC and PI and subjected to flow cytometry. The percentage of cells exhibiting necrosis (PI-positive cells, panel 1) and apoptosis (annexin V-FITC-positive and PI-negative, panel 2) are shown. **, p < 0.01, when compared with time 0.