FIGURE 5.

Neuroprotective effect of Nec-1 against 24S-OHC-induced cell death. A, SH-SY5Y cells were exposed to 24S-OHC in the absence or presence of autophagy inhibitors (10 mm 3-methyladenine (3MA), 20 μm LY294002 (LY), and 100 nm wortmannin (Wm)) for 24 h, and the viability was measured by LDH assay. B, SH-SY5Y cells (panel 1) and cultured cortical neuronal cells (panel 2) were exposed to 24S-OHC in the absence or presence of Nec-1 for 24 h, and the viability was measured by LDH assay. **, p < 0.01, when compared with vehicle control (without Nec-1).