FIGURE 8.

Cell death and activation of caspase-3 induced by 24S-OHC in Jurkat cells. A, cell death induced by 24S-OHC in Jurkat cells. The cells were treated with variable amounts of 24S-OHC for 24 h, and the viability was measured by MTT assay. **, p < 0.01, when compared with vehicle control (without 24S-OHC). B, Western blot analysis of caspase-3 in 24S-OHC-treated Jurkat cells. The cells were treated with 24S-OHC or STS or H₂O₂ for the indicated times, and then the cell samples were subjected to Western blot analysis using an antibody against caspase-3.