FIGURE 3.

Lysosome area and polygon length are increased in BI-1-overexpressing cells. A, Neo and BI-1 cells were exposed to 5 μm LysoTracker and photographed. Fluorescence intensity was quantified (right). B, lysosome volume per cell was also analyzed and quantified. C and D, electron microscopy analysis was performed (C), and the lysosomal area and polygonal length were quantified (D). The arrow in panel C indicates lysosomes. *, p < 0.05; significantly different from Neo cells. In panels A, B, and D, each value is expressed as the mean ± S.E. of three independent experiments.