FIGURE 8.

BAX/BAK dependence of BH3 mimetic-induced apoptosis. A, wild-type Jurkat and JurkatΔBAK cells were incubated with each of the BH3 mimetics for 0–72 h, stained with Hoechst 33342, and scored for chromatin condensation. The results reflect the average of three independent experiments at most time points. The S.E. values are presented where n = 3, and they exceed the size of the symbol. B, wild-type Jurkat and JurkatΔBAK cells were incubated with 0.3 μm ABT-737 plus gossypol, S1, or 2-methoxy-antimycin A₃ for 6 h, stained with Hoechst 33342, and scored for chromatin condensation. The cells were also incubated with ABT-737, gossypol, S1, or 2-methoxy-antimycin A₃ for 6 h and lysed for Western blot analysis.