FIGURE 7.

Effects of WT and dominant negative forms of DUSP16 on antigen-specific IFNγ and IL-4 production by CD4⁺ T cells. CD4⁺ T cells were isolated from immunized mice and cultured in vitro for 3 days. Then, cells were collected and stimulated with indicated concentrations of antigen (OVA). Production of IFNγ (A) and IL-4 (B) in the supernatants of antigen-stimulated cells was determined by ELISA. Data are mean ± S.D. (error bars) from three mice/group and are representative of three independent experiments. n.d., not detected.