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. 2011 May 12;286(28):24931–24942. doi: 10.1074/jbc.M111.241208

FIGURE 1.

FIGURE 1.

M13mp2 phage dsDNA constructs having an ssDNA gapped region containing trinucleotide motif cassettes inserted in-frame within a lacZα mutational reporter gene. a, 32 AGC hot motifs on the left-hand side and 24 AGC hot motifs on the right-hand side separated by a 9-nt linker region. b–d, each construct contains an identical cassette composed of alternating AAC hot and AGC hot′ motifs on the left-hand side and two cassettes on the right-hand side containing AAC hot motifs and GAC neutral motifs (b and c) or one cassette on the right-hand side containing AAC hot motifs and GTC cold motifs (d). AID deaminations within the inserted cassettes create stop codons in the lacZα reading frame of M13 phage progeny resulting in colorless mutant plaques. Unmutated gap molecules give rise to “wild type” dark blue plaques. Deaminations in the inserted cassettes are detected as C→T mutations by sequencing individual DNA clones isolated from the mutant colorless phage plaques.