FIGURE 2.

AID-initiated mutations in AGC₃₂-AGC₂₄ and (AAC AGC)₁₅-(AAC GAC)₁₅-(AAC GAC)₁₅ measured as a function of time. a, percentage of clones having 1–4 or ≥5 mutations for exposures to AID ranging from 15 s to 5 min. b, average numbers of mutations per clone accumulating with time. The average mutation rates are 1 per 46 s in AGC₃₂-AGC₂₄ and 1 per 63 s in (AAC AGC)₁₅-(AAC GAC)₁₅-(AAC GAC)₁₅. The incubation conditions, including AID and ssDNA concentrations, are described under “Experimental Procedure.”