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Mutation spectra for alternating hot-hot′ and hot-cold, alternating hot-hot′ and hot-neutral, and irregular hot-hot′ and hot-neutral constructs. The composition of each cassette is shown below the bar graphs. The bars give the percent of clones with mutations at each motif position, AAC (red), AGC (orange), GTC (blue), and GAC (green). A mutation in the spacer region in an ATC motif is shown in black.
