TABLE 2.
Distance between mutated motifs in clones with 2–10 mutations in the combined (AAC AGC)15 cassettes
| Mutations/clone | Distribution of distance between mutated motifs (%) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | 5–10 | 11–15 | 16–20 | 21–29 | ||
| 2 | Data (209)a | 2.4 | 11.0 | 5.7 | 9.1 | 4.8 | 27.8 | 19.1 | 12.4 | 7.7 |
| 2 | Simulatedb | 8.5 | 9.0 | 6.7 | 7.9 | 6.1 | 26.3 | 16.2 | 11.6 | 7.7 |
| 3 | Data (113) | 8.8 | 8.8 | 5.3 | 9.3 | 8.4 | 31.4 | 20.4 | 5.8 | 1.8 |
| 3 | Simulated | 11.5 | 11.4 | 9.1 | 9.0 | 6.9 | 27.9 | 14.5 | 7.0 | 2.7 |
| 4 | Data (64) | 14.6 | 14.6 | 6.8 | 8.9 | 6.3 | 31.8 | 16.1 | 1.0 | 0.0 |
| 4 | Simulated | 13.8 | 14.5 | 10.4 | 10.7 | 7.5 | 27.1 | 11.3 | 3.9 | 0.8 |
| 5 | Data (55) | 15.9 | 17.7 | 9.5 | 12.7 | 6.4 | 25.9 | 10.5 | 1.4 | 0.0 |
| 5 | Simulated | 17.3 | 17.0 | 11.4 | 11.6 | 7.8 | 24.8 | 8.0 | 2.0 | 0.2 |
| 6 | Data (37) | 15.1 | 20.5 | 8.6 | 11.4 | 16.2 | 23.8 | 3.8 | 0.5 | 0.0 |
| 6 | Simulated | 20.3 | 18.8 | 12.8 | 11.6 | 8.0 | 22.1 | 5.4 | 0.9 | 0.0 |
| 7 | Data (27) | 16.7 | 23.5 | 13.6 | 8.6 | 7.4 | 27.8 | 2.5 | 0.0 | 0.0 |
| 7 | Simulated | 23.4 | 20.8 | 13.3 | 12.0 | 7.8 | 18.9 | 3.5 | 0.4 | 0.0 |
| 8 | Data (19) | 21.8 | 21.8 | 11.3 | 12.0 | 12.0 | 20.3 | 0.8 | 0.0 | 0.0 |
| 8 | Simulated | 26.5 | 22.7 | 14.0 | 11.8 | 7.1 | 15.6 | 2.1 | 0.1 | 0.0 |
| 9 | Data (16) | 25.8 | 27.3 | 12.5 | 9.4 | 5.5 | 19.5 | 0.0 | 0.0 | 0.0 |
| 9 | Simulated | 29.5 | 24.2 | 14.4 | 11.4 | 6.7 | 12.7 | 1.2 | 0.0 | 0.0 |
| 10 | Data (12) | 30.6 | 25.9 | 12.0 | 13.9 | 5.6 | 12.0 | 0.0 | 0.0 | 0.0 |
| 10 | Simulated | 32.9 | 25.1 | 14.2 | 10.9 | 6.0 | 10.2 | 0.6 | 0.0 | 0.0 |
a Numbers in parentheses are the number of sequenced clones.
b Simulated refers to the modified random walk model (see text) with the following parameters: AID-catalyzed deamination efficiency in AAC motif = 3%; average slide distance obtained from geometric distribution = 30 nt (10 motifs).