FIGURE 5.
SPR data for the interaction of C461A-cDsbD with nDsbDox. a, representative sensorgrams for C461A-cDsbD binding to nDsbDox coupled to the chip surface. b, representative sensorgrams for the reference cell. The concentration of C461A-cDsbD in a and b ranged from 44 to 525 μm as indicated. c, the observed response, the difference between the response in a and b at equilibrium, is plotted as a function of the concentration of C461A-cDsbD flowed over a chip to which nDsbDox was coupled. A Kd value of 0.086 mm is obtained from a fit to these data (solid curve). d, the SPR data give a linear Scatchard plot. The solid line was calculated using the Kd value fitted in c.