FIGURE 2.

G2L3 is expressed ubiquitously in human tissues and cell lines. A, PCRs were performed on cDNA panels generated from different tissues, using primers specific for G2L3, which spanned exons 2 to 6. Primers designed specifically to recognize β-actin were used as loading controls. B, PCRs were performed using cDNA libraries generated from different cell lines. The human tissues, human cell lines (HeLa, HT1080, human foreskin fibroblast (HFF), and HEK293) and mouse cell line (NIH3T3) are indicated above each panel, the sizes of DNA are indicated on the left side of the panel in bp, and the expected PCR product sizes are indicated by arrowheads. Note the low expression of G2L3 in brain tissue and its absence in the HT1080 cell line. The data are representative of three independent experiments.