FIGURE 5.

Neither the integrity of actin nor that of microtubules is a prerequisite for G2L3 localization to microtubules or actin cytoskeletons, respectively. A, NIH3T3 cells expressing G2L3 were treated with either 10 μm nocodazole (NOC) or 2 μm cytochalasin D (CYTO D) for 30 min to determine their effects on G2L3 localization. Note that G2L3 can localize to stable MTs (top row, black arrow) and actin filaments (top row, black arrowheads) after NOC treatment, whereas G2L3 predominantly localizes to MTs (bottom row, black arrow) after treatment of cells with cytochalasin D. B, the subcellular localization of G2L3 in cells was quantified after treatment with nocodazole or cytochalasin D. Note that G2L3 localizes diffusely in a greater proportion of cells after NOC treatment, whereas G2L3 localizes to MTs in more cells after cytochalasin D (Cyto D) treatment. More than 50 cells were counted for each treatment and are representative of three independent experiments. Bars, 10 μm.