FIGURE 3.

TTP and dUTP incorporation rates of HIV-1 RT in macrophage conditions. A, single nucleotide extension reaction time-courses using the same template-primer (P) used in Fig. 2 were performed in vitro with the reconstituted TTP and dUTP concentrations found in macrophages. E, extended product. B, product formation for TTP (black line), dUTP (blue line), and both TTP and dUTP (green line) were plotted. C, the rates of the product formation (B) were determined with a double exponential equation as described under “Experimental Procedures.” A 20 nm final concentration of HIV-1 RT was used to initiate the reaction in which small aliquots were obtained over the time course indicated. The experiments were repeated in triplicate.