FIGURE 5.

Effect of ddU treatment on HIV-1 replication in primary human macrophages and activated CD4⁺ T cells. Primary human macrophage were pretreated with ddU and then transduced with a single round vesicular stomatitis virus glycoprotein pseudotyped HIV-1 vector (D3HIV). A, the normalized vector transduction efficiency in macrophages at the indicated ddU concentrations from n = 5 independent human donors. B, FACS analysis for the macrophages in A for GFP-expressing cells. C, the normalized vector transduction efficiency in human primary activated CD4⁺ T cells at the indicated ddU concentrations from three independent human donors. D, 2LTR circle quantitative PCR plotted by ddU concentration from four independent human donors. E, ddU toxicity assessed by the live and dead cell assay for primary human lung fibroblasts (MRC5), human microglial cell line (CHME5), human primary activated CD4⁺ T cells, and primary human macrophage.