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. 2011 May 11;286(28):25056–25064. doi: 10.1074/jbc.M110.213777

FIGURE 1.

FIGURE 1.

Purified CCM3 and GCKIII proteins interact directly. A, domain organization of CCM3 and GCKIII proteins. ND, N-terminal dimerization region of CCM3. B, in vitro interaction between GST fusions of STK25 and MST4 with CCM3. The minimal MST4 kinase domain does not bind CCM3. C, CCM3 interacts with the C-terminal tail regions of MST4 and STK25 but not MST1. The CCM3 C-terminal FAT domain does not interact with the kinases. D, the amino terminus of CCM3 (CCM3-ND) is sufficient for interaction with full length MST4. E, CCM3-ND is sufficient for interaction with the C-terminal tail region of STK25. F, CCM3 binds tightly to the GST-STK25 tail but not to GST-CCM3.